Small molecule bioanalysis refers to the scientific process of quantifying and analyzing low molecular weight compounds, typically drugs or metabolites, in biological samples such as blood, plasma, urine, or tissue. This field is essential in pharmaceutical research and development, as well as in clinical diagnostics, as it provides crucial insights into drug pharmacokinetics, metabolism, efficacy, and safety profiles. Small molecule bioanalysis employs advanced analytical techniques such as liquid chromatography-mass spectrometry (LC-MS) or gas chromatography-mass spectrometry (GC-MS) to accurately measure and characterize the concentration and distribution of small molecules in biological matrices. These analyses play a critical role in drug discovery, preclinical and clinical studies, therapeutic drug monitoring, and regulatory submissions, ensuring the safety and effectiveness of pharmaceutical products.